Research interest

Our aim is to develop and apply novel super resolution microscopy approaches to contribute to new discoveries and ideas within the basic life sciences. Our long-term goal is to contribute to the understanding of fundamental biological processes relevant for health and disease.

Super Resolution

Fluorescence microscopes, and especially their confocal and two-photon variants, are unique in their ability to observe directly morphological changes and molecular reactions in living cells. However, they are limited in resolution by the diffraction barrier (about 200-300 nm). This limitation is overcome with great success by the field of super-resolution microscopy...


Utilizing both the low light intensities of RESOLFT combined with the high acquisition speeds of STED we study neuronal protein organization and dynamics in brain tissues. We continuosly push the spatial and temporal resolution of novel microscopy techniques to enable more in depth studies of nanoscale structures and dynamics in biological samples.

Progress in science depends on new techniques, new discoveries and new ideas, probably in that order.

Sydney Brenner

Selected Publications

Three dimensional parallelized RESOLFT nanoscopy for volumetric live cell imaging, bioRxiv (Nature Biotechnology accepted, in press) (2020)

Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo, Nature communications 10, 556 (2019)

Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems, Nature Communications 9, 3281 (2018)

Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy, Nature methods 15, 601-604 (2018)

Dual Channel RESOLFT Nanoscopy by Using Fluorescent State Kinetics, Nano Letters 15, 103-106 (2015)

CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells. Scientific Reports 5, 9592 (2015)

Nanoscopy of living brain slice with low light levels, Neuron 75: 992–1000 (2012)

Diffraction-unlimited all-optical imaging and writing with a photochromic GFP, Nature 478: 204-208 (2011)

About SciLifeLab

Our lab is located in the Science for Life Laboratory (SciLifeLab), a national center for molecular biosciences with focus on health and environmental research. SciLifeLab has been created by the coordinated effort of four universities in Stockholm and Uppsala: Stockholm University, Karolinska Institutet, KTH Royal Institute of Technology and Uppsala University.

Open Positions

We are looking for self-motivated and curiosity-driven candidates with an expertise in physics, chemistry or biology, who are looking to make original contributions to the field of super-resolution microscopy. The candidates will work on collaborative projects investigating the nanoscale organization and dynamics of proteins in living neurons and brain tissue with our cutting edge microscopy technology. We offer an outstanding scientific environment and a vibrant working climate with individual freedom and various possibilities for professional development. The work will be funded by the European Union within the ERC project “MoNaLISA”.

Get in Touch

    Ilaria Testa, PhD.
    Associate Professor
    KTH Royal Institute of Technology
    Dept. of Applied Physics
  • Email:
  • Address:
    Science for Life Laboratory
    Tomtebodavägen 23A
    171 65 Stockholm

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