Research interest
Our aim is to develop and apply novel super resolution microscopy approaches to contribute to new discoveries and ideas within the basic life sciences. Our long-term goal is to contribute to the understanding of fundamental biological processes relevant for health and disease.
Super Resolution
Fluorescence microscopes, and especially their confocal and two-photon variants, are unique in their ability to observe directly morphological changes and molecular reactions in living cells. However, they are limited in resolution by the diffraction barrier (about 200-300 nm). This limitation is overcome with great success by the field of super-resolution microscopy...
Applications
Utilizing both the low light intensities of RESOLFT combined with the high acquisition speeds of STED we study neuronal protein organization and dynamics in brain tissues. We continuosly push the spatial and temporal resolution of novel microscopy techniques to enable more in depth studies of nanoscale structures and dynamics in biological samples.
Progress in science depends on new techniques, new discoveries and new ideas, probably in that order.
Sydney Brenner
Selected Publications
Three dimensional parallelized RESOLFT nanoscopy for volumetric live cell imaging, bioRxiv (Nature Biotechnology accepted, in press) (2020)
Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo, Nature communications 10, 556 (2019)
Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems, Nature Communications 9, 3281 (2018)
Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy, Nature methods 15, 601-604 (2018)
Dual Channel RESOLFT Nanoscopy by Using Fluorescent State Kinetics, Nano Letters 15, 103-106 (2015)
CRISPR/Cas9-mediated endogenous protein tagging for RESOLFT super-resolution microscopy of living human cells. Scientific Reports 5, 9592 (2015)
Nanoscopy of living brain slice with low light levels, Neuron 75: 992–1000 (2012)
Diffraction-unlimited all-optical imaging and writing with a photochromic GFP, Nature 478: 204-208 (2011)